It contains mem α 20 fbs 1 pen strep and a small amount of 2 mercaptoethanol.
Cell culture media protocol.
As the cells are in 100 ml media the next calculation is.
Cell line passage number etc.
Basal media mem α provides the basic nutrients that cells need such as amino acids ions and a ph buffer.
2 a semi synthetic solid medium containing sucrose as c source and nitrate as the sole source of nitrogen useful for the general cultivation of fungi yeasts and soil bacteria.
Cell culture basics techniques and media essentially cell culture involves the distribution of cells in an artificial environment in vitro which is composed of the necessary nutrients ideal temperature gases ph and humidity to allow the cells to grow and proliferate.
Most cell lines can be grown using dmem culture media or rpmi culture media with 10 foetal bovine serum fbs 2 mm glutamine and antibiotics can be added if required see table below.
In vivo when the study involves living biological entities within the organism.
If the cell count is 55 x 10 4 ml and there is 100 ml of cell suspension.
Suitable for cell culture.
Label culture flask with all necessary info e g.
This will give a value for the volume of media the cells should be in.
Then resuspend cells in sterile media to a suitable volume for counting.
2 cryopreservation 2 for cell culture.
Based on count and viability data seed cell suspension for an appropriate flask and density e g.
Check which culture media and culture supplements the cell line you are using requires before starting cultures.
Cell culture cell culture is one of the major tools used in cellular and molecular biology providing excellent model systems for studying the normal physiology and biochemistry of cells e g metabolic studies aging the effects of drugs and toxic compounds on the cells.
55 x 10 4 cells ml 1 22 45.
9 cell culture antibiotic sterile filtered.
Add the correct amount of pre warmed culture media using serological pipette.
100 ml x dilution factor.
This protocol is for making complete media suitable for culturing tenocytes tendon cells.
